Fluctuations of Prunus Necrotic Ringspot Virus (PNRSV) at Various Phenological Stages in Peach Cultivars
نویسندگان
چکیده
منابع مشابه
Comparison of DAS-ELISA and RT-PCR Methods for the Detection of Prunus Necrotic Ringspot Virus (PNRSV)
Two methods (DAS-ELISA and RT-PCR) were compared for the detection of Prunus necrotic ringspot virus (PNRSV) in woody host Prunus mahleb isolated from Malatya. Total RNA extractions were made from serially diluted fresh leaf tissue, root, bark and one year old green bark tissue using silica-based method. Purified total RNA extracts were used as template for cDNA synthesis by reverse transcripti...
متن کاملAnalysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing
PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved g...
متن کاملExpression of Prunus Necrotic Ringspot Virus Coat Protein in E. coli
Background and Amis: Serological assay is considered as one of the best choices for conducting large number of infection tests. Recombinant DNA technology has been used for expression of virus coat protein (CP) gene in prokaryotic bacterial cells such as Escherichia coli and the recombinant CP (rCP) is used as immunogen in antibody production. Heterologous CP protein expression and purification...
متن کاملWhole-Genome Characterization of Prunus necrotic ringspot virus Infecting Sweet Cherry in China
Prunus necrotic ringspot virus (PNRSV) causes yield loss in most cultivated stone fruits, including sweet cherry. Using a small RNA deep-sequencing approach combined with end-genome sequence cloning, we identified the complete genomes of all three PNRSV strands from PNRSV-infected sweet cherry trees and compared them with those of two previously reported isolates.
متن کاملVertical transmission of Prunus necrotic ringspot virus: hitch-hiking from gametes to seedling.
The aim of this work was to follow Prunus necrotic ringspot virus (PNRSV) infection in apricot reproductive tissues and transmission of the virus to the next generation. For this, an analysis of viral distribution in apricot reproductive organs was carried out at different developmental stages. PNRSV was detected in reproductive tissues during gametogenesis. The virus was always present in the ...
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ژورنال
عنوان ژورنال: Plant Disease
سال: 1999
ISSN: 0191-2917,1943-7692
DOI: 10.1094/pdis.1999.83.11.1055